ENA - Extractable Nuclear Antigens


Connective tissue disease (CTD) is a common autoimmune disease. The connective tissues are structural portions of the body such as collagen and elastin which are essential to provide a framework or matrix for the body.  In patients with CTD, it is common for collagen and elastin to become injured by inflammation.  We have a useful part to play in the diagnosis and monitoring of patients with connective tissue disease. We have a CTD service which tests for systemic lupus erythematosus, rheumatoid arthiritis, scleroderma, polymyositis and dermatomyositis amongst many others. The CTD service provides the following test panel to aid in diagnosis:


Tests available are: Anti-Nuclear Antibody (ANA), Complement C3 and C4 (C34), Extractable Nuclear Antigen (ENA) and double stranded Deoxyribonucleic acid (DNA) antibody.

Judicious use of these tests in conjunction with clinical history and examination can help in the diagnosis and monitoring of CTD.

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Extractable Nuclear Antigens (ENA)


Immunology Laboratory

Specimen Type:



If samples have to be stored prior to dispatch, please keep at 4°C

Posting Details:

Room temperature

Posting Address:

Department of Immunology, City Hospital, Dudley Road, Birmingham, B18 7QH

Clinical Use:

Antibodies to extractable nuclear antigens are found in various connective tissue conditions, e.g. SLE, Sjogrens syndrome


Up to 7 days for ENA screen, up to 14 days for ENA profile

External QA Scheme:

UK NEQAS, antibodies to nuclear and related antigens

CPA Accredited Lab/Test:

Full accreditation

Key Contact:

Shabir Rajpar

Email: info@cityassays.org.uk

Tel: 0121 507 4258

Clinical Lead for this assay:

Dr Sadia Nooorani

Tel: 0121 507 5157






  SWBH NHS Trust 2018